A three-day course in Industrial Biotechnology and Protein Engineering – with presentation by GlycoSpot

At Hotel Phoenix Copenhagen in the Heart of Copenhagen, Denmark

Industrial training addressing technicians as well as process engineers working in the chemical, pharmaceutical, biotech and/or food industry

Target group & outcome

Take this course if you want to understand the background of the development and production of biotech products. Take the course if you are a non-biotechnologist who need to understand and communicate with your R&D colleagues and/or if you need to brush up your current knowledge about molecular biology and biotechnology.

The course runs for three consecutive days with alternating presentations, exercises and plenum discussions. The course is held in English.

Main topics taught in the course

  • The cell and cell types
  • Protein function and stability
  • Enzymes
  • DNA and the gene
  • Industrial protein engineering and how to design a protein
  • Industrial use of enzymes
  • New enzyme assay technology for industrial use
  • Industrial production of proteins
  • Plenum exercises and discussions

Detailed agenda (see more info in the Course flyer)

Day 1:

Subject

10.00 – 10.15 Course introduction, participant introduction and setting the scene for expectations.
10.15 – 12.00 The Cell – a small factory: The samllest living unit. Different cell types, prokaryotes vs. eukaryotes. Organisation of the cell, the nucleus and organelles.
12.00 – 13.00 Lunch
13.00 – 15.15 Understanding protein function and stability: what is a protein. the structure of proteins. what is keeping proteins together or making them fall apart. Factors defining protein stability. Folding, modification and degradation of proteins. Protein function.
15.15 – 16.00 Enzymes: what is an enzyme. Enzyme specificity and activity. Where are enzymes used industrially.
16.30 – 21.30 Course dinner in Nyhavn

Day 2:

Subject

9.00 – 10.30 DNA and the gene: what is DNA. Definition of a gene. DNA replication.
10.30 – 12.00 Translating DNA into a functional protein: the genetic code – the code of life. Transcription and translation. Protein synthesis.
12.00 – 13.00 Lunch
13.00 – 16.00 Industrial protein engineering; How to design a protein: cloning and recombination. Producing DNA – the polymerase chain reaction (PCR). Site directed mutagenesis. Random mutagenesis. Case study – optimisation of an enzyme.

Day 3:

Subject

9.00 – 10.00 Industrial protein engineering: sequence analysis of cloned DNA. Bioinformatics.
10.00 – 11.00 Industrial use of enzymes: classification of enzymes. Enzyme applications. Safety and regulations.
11.00 – 12.00 New enzyme assay technologies for industrial use. Presented by Glycospot
12.00 – 13.00 Lunch.
13.00 – 15.00 Industrial production of proteins: using single cells as small protein factories. Intracellular vs. extracellular expression. Fungal expression. Bacterial and mammalian expression
15.00 – 15.45 Wrap up – connecting all the dots and drawing the full picture.
16.00 Final remarks, Course diplomas, Course evaluation.

Teachers and venue

Pernille Ollendorff Hede, M.Sc. (Biochemistry), Ph.D. Ditte Marie Kjær Lomholt, M.Sc. (Biochemistry)
  • More than 10 years of experience within teaching biotechnology to non-biotechnologists
  • More than 10 years of experience within teaching biotechnology to non-biotechnologists
Guest speakers:

Hotel Phoenix Copenhagen is a 4-star deluxe hotel housed in one of Copenhagens historic buildings. Situated in central Copenhagen, near Amalienborg Palace, just a few metres walk from Nyhavn, Kongens Nytorv, Strøget and other sights.

Address: Bredgade 37, DK-1260 Copenhagen K, Denmark

Telephone: + 45 33 95 95 00

Booking & service: [email protected]

 

Fee and registration

The course fee is payable in advance and includes course materials, calculator, coffee & refreshments, lunch all three days and dinner on the first afternoon.

Per delegate EUR 1945,- plus VAT. VAT is reclaimable.

Overnight stay at the delegates own expense can be arranged at the course venue or elsewhere nearby. Please contact Hotel Phoenix Copenhagen at +45 33 95 95 00 or [email protected].

Kindly note that Copenhagen is a very popular destination and that Copenhagen hotels may be fully booked well in advance

Binding registration at www.seydlitz.dk/courses no later than 1st of March 2021. In case of any questions please contact [email protected] or at +45 44 10 87 00

The course may be subject to cancellation in case of too few participants.